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📝 The Mitochondrial L-Lactate Dehydrogenase Affair

The existence of a mitochondrial L-lactate dehydrogenase (m-L-LDH) suggested by Dianzani (1951), was shown by Baba and Sharma (1971) with the enzyme located in the mitochondrial matrix; later Brooks et al. (1999) proposed the intracellular lactate shuttle and in the third millennium the existence of m-L-LDH was definitively been confirmed in mammalian, plant and yeast mitochondria as reviewed by Schurr (2006)Passarella et al. (2008), and Brooks (2009), being its existence finally recognized by inclusion of m-L-LDH in the Mitocarta (http://www.broadinstitute.org/pubs/MitoCarta/index.html). The experimental strategy to be used to show whether and how L-lactate can enter mitochondria to be metabolized is well-established and has been applied to a variety of mitochondria including heart (Brooks et al., 1999Valenti et al., 2002), liver (Brooks et al., 1999de Bari et al., 2004), skeletal muscle (Dubouchaud et al., 2000de Bari et al., 2008Passarella et al., 2008) plant (Paventi et al., 2007), brain (Schurr, 2006Atlante et al., 2007Schurr and Payne, 2007Hashimoto et al., 2008), and cancer cells (de Bari et al., 2010aPizzuto et al., 2012). Thus, it is a matter for considerable surprise that the overwhelming evidence for an m-L-LDH located inside mitochondria is not by now universally accepted (Rasmussen et al., 2002Sahlin et al., 2002Ponsot et al., 2005Gladden, 2007Yoshida et al., 2007Elustondo et al., 2013).

Updated on July 13, 2020

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